› 5nm3

X-ray diffraction
3.3Å resolution

Deinococcus radiodurans BphP PAS-GAF-PHY Y263F mutant, pre-illuminated

Released:
DOI: 10.2210/pdb5nm3/pdb
PDB entry 5nm3 coloured by chain, front view.
PDB entry 5nm3 coloured by chain, side view.
PDB entry 5nm3 coloured by chain, top view.
Source organism: Deinococcus radiodurans R1
Primary publication:
On the (un)coupling of the chromophore, tongue interactions, and overall conformation in a bacterial phytochrome.
Takala H, Lehtivuori HK, Berntsson O, Hughes A, Nanekar R, Niebling S, Panman M, Henry L, Menzel A, Westenhoff S, Ihalainen JA
J Biol Chem 293 8161-8172 (2018)
PMID: 29622676

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193367 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophytochrome Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 523 amino acids
Theoretical weight: 56.57 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RZA4 (Residues: 1-502; Coverage: 67%)
Gene names: DR_A0050, bphP
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand LBV 4 x LBV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 85.7Å b: 198.5Å c: 223.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.234 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Bacteriophytochromes - from informative model systems of phytochrome function to powerful tools in cell biology.
Gourinchas et al. (2019)
1 more

1 mention without citation

On the (un)coupling of the chromophore, tongue interactions, and overall conformation in a bacterial phytochrome.
Takala et al. (2018)