5nm3

X-ray diffraction
3.3Å resolution

Deinococcus radiodurans BphP PAS-GAF-PHY Y263F mutant, pre-illuminated

Released:

Function and Biology Details

Reaction catalysed:
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophytochrome Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 523 amino acids
Theoretical weight: 56.57 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RZA4 (Residues: 1-502; Coverage: 67%)
Gene names: DR_A0050, bphP
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 85.7Å b: 198.5Å c: 223.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.234 0.267
Expression system: Escherichia coli BL21(DE3)