5nkk

X-ray diffraction
2.64Å resolution

SMG8-SMG9 complex GDP bound

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nonsense-mediated mRNA decay factor SMG8 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 429 amino acids
Theoretical weight: 49.78 KDa
Source organism: Caenorhabditis elegans
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O62301 (Residues: 1-423; Coverage: 49%)
Gene names: K04B12.3, smg-8
Sequence domains: Smg8_Smg9
Nonsense-mediated mRNA decay factor SMG9 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 317 amino acids
Theoretical weight: 36.81 KDa
Source organism: Caenorhabditis elegans
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9XWJ1 (Residues: 59-375; Coverage: 82%)
Gene names: Y54E2A.2, smg-9

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 110.605Å b: 110.605Å c: 360.266Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.232 0.274
Expression system: Spodoptera frugiperda