PDBe 5nj6

X-ray diffraction
4Å resolution

Crystal structure of a thermostabilised human protease-activated receptor-2 (PAR2) in ternary complex with Fab3949 and AZ7188 at 4.0 angstrom resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proteinase-activated receptor 2, alternate cleaved 2; Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 437 amino acids
Theoretical weight: 49.21 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
  • Canonical: P55085 (Residues: 55-269, 276-377; Coverage: 85%)
  • nullnull
Gene names: F2RL1, GPR11, PAR2, cybC
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Fab3949 H Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 23.59 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Fab3949 L Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.32 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 164.144Å b: 38.52Å c: 159.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.263 0.319
Expression systems:
  • Spodoptera frugiperda
  • Mus musculus