› 5nih

X-ray diffraction
1.3Å resolution

Crystal structure of the GluA2 ligand-binding domain (S1S2J) in complex with agonist LM-12b at 1.3 A resolution.

Released:
DOI: 10.2210/pdb5nih/pdb
PDB 5nih coloured by chain and viewed from the front.
PDB 5nih coloured by chain and viewed from the side.
PDB 5nih coloured by chain and viewed from the top.
Source organism: Rattus norvegicus
Primary publication:
Structure and Affinity of Two Bicyclic Glutamate Analogues at AMPA and Kainate Receptors.
Møllerud S, Pinto A, Marconi L, Frydenvang K, Thorsen TS, Laulumaa S, Venskutonytė R, Winther S, Moral AMC, Tamborini L, Conti P, Pickering DS, Kastrup JS
ACS Chem Neurosci 8 2056-2064 (2017)
PMID: 28691798

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 29.28 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19491 (Residues: 413-527, 653-797; Coverage: 30%)
Gene names: Glur2, Gria2
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

5 bound ligands:
Ligand SO4 5 x SO4
Ligand 8VE 2 x 8VE
Ligand GOL 4 x GOL
Ligand CL 2 x CL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 62.577Å b: 87.954Å c: 96.535Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.128 0.127 0.158
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Membrane-protein crystals for neutron diffraction.
Sørensen et al. (2018)
1 more