5nhs

X-ray diffraction
2.11Å resolution

The crystal structure of Xanthomonas albilineans N5, N10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)

Released:
Entry authors: Bueno RV, Guido RVC, Maluf FV, Lima GMA, Oliveira AA

Function and Biology Details

Reactions catalysed:
5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate
5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein FolD Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 31.46 KDa
Source organism: Xanthomonas albilineans GPE PC73
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D2UAL4 (Residues: 1-296; Coverage: 100%)
Gene names: XALc_1581, folD
Sequence domains:

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P21212
Unit cell:
a: 123.295Å b: 76.404Å c: 66.641Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.241
Expression system: Escherichia coli BL21(DE3)