› 5nhn

X-ray diffraction
1.96Å resolution

Super-Folder Green Fluorescent Protein Artificiall dimer linked via 148 position

Released:
DOI: 10.2210/pdb5nhn/pdb
PDB 5nhn coloured by chain and viewed from the front.
PDB 5nhn coloured by chain and viewed from the side.
PDB 5nhn coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Primary publication:
Positive functional synergy of structurally integrated artificial protein dimers assembled by Click chemistry
Worthy HL, Auhim HS, Jamieson WD, Pope JR, Wall A, Batchelor R, Johnson RL, Watkins DW, Rizkallah PJ, Castell OK, Jones DD
Commun Chem (2019)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Green fluorescent protein Chain: B
Molecule details ›
Chain: B
Length: 228 amino acids
Theoretical weight: 25.82 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 3-231; Coverage: 96%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 25.84 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 3-231; Coverage: 96%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand DB5 1 x DB5
Ligand GOL 1 x GOL
Ligand EDO 1 x EDO
1 modified residue:
Ligand CRO 2 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P65
Unit cell:
a: 99.8Å b: 99.8Å c: 108.92Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.18 0.209
Expression system: Escherichia coli

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