› 5nh3

X-ray diffraction
2.35Å resolution

CRYSTAL STRUCTURE OF THE Activin receptor type-2A LIGAND BINDING DOMAIN IN COMPLEX WITH BIMAGRUMAB FV

Released:
DOI: 10.2210/pdb5nh3/pdb
PDB entry 5nh3 coloured by chain, front view.
PDB entry 5nh3 coloured by chain, side view.
PDB entry 5nh3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Blockade of activin type II receptors with a dual anti-ActRIIA/IIB antibody is critical to promote maximal skeletal muscle hypertrophy.
Morvan F, Rondeau JM, Zou C, Minetti G, Scheufler C, Scharenberg M, Jacobi C, Brebbia P, Ritter V, Toussaint G, Koelbing C, Leber X, Schilb A, Witte F, Lehmann S, Koch E, Geisse S, Glass DJ, Lach-Trifilieff E
Proc Natl Acad Sci U S A 114 12448-12453 (2017)
PMID: 29109273

Function and Biology Details

Reaction catalysed: 
ATP + [receptor-protein] = ADP + [receptor-protein] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210931 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Activin receptor type-2A Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 14.2 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P27037 (Residues: 20-135; Coverage: 24%)
Gene names: ACVR2, ACVR2A
Sequence domains: Activin types I and II receptor domain
Structure domains: CD59
anti-human ActRIIB mAb BYM338 Fv heavy-chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 121 amino acids
Theoretical weight: 13.41 KDa
Source organism: Homo sapiens
anti-human ActRIIB mAb BYM338 Fv light-chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 119 amino acids
Theoretical weight: 12.51 KDa
Source organism: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 44.33Å b: 108.207Å c: 100.685Å
α: 90° β: 98.25° γ: 90°
R-values:
R R work R free
0.168 0.166 0.208
Expression system: Homo sapiens

Quick links

Citations

22 review citations

Role of Myokines in Regulating Skeletal Muscle Mass and Function.
Lee et al. (2019)
21 more

6 mentions without citation

Blockade of activin type II receptors with a dual anti-ActRIIA/IIB antibody is critical to promote maximal skeletal muscle hypertrophy.
Morvan et al. (2017)
5 more