› 5ngd

X-ray diffraction
1.9Å resolution

Crystal structure of the V325G mutant of the bacteriophage G20C portal protein

Released:
DOI: 10.2210/pdb5ngd/pdb
PDB entry 5ngd coloured by chain, front view.
PDB entry 5ngd coloured by chain, side view.
PDB entry 5ngd coloured by chain, top view.
Source organism: Thermus virus P74-26
Primary publication:
Porphyrin-Assisted Docking of a Thermophage Portal Protein into Lipid Bilayers: Nanopore Engineering and Characterization
Cressiot B, Greive SJ, Si W, Pascoa TC, Mojtabavi M, Chechik M, Jenkins HT, Lu X, Aksimentiev A, Antson AA, Wanunu M
ACS Nano 11 11931-11945 (2017)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Portal protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 417 amino acids
Theoretical weight: 45.98 KDa
Source organism: Thermus virus P74-26
Expression system: Escherichia coli
UniProt:
  • Canonical: A7XXR3 (Residues: 25-438; Coverage: 92%)
Gene name: P74p85

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: R3
Unit cell:
a: 223.234Å b: 223.234Å c: 115.817Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.225
Expression system: Escherichia coli

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