› 5nfq

X-ray diffraction
1.6Å resolution

Novel epoxide hydrolases belonging to the alpha/beta hydrolases superfamily in metagenomes from hot environments

Released:
DOI: 10.2210/pdb5nfq/pdb
PDB 5nfq coloured by chain and viewed from the front.
PDB 5nfq coloured by chain and viewed from the side.
PDB 5nfq coloured by chain and viewed from the top.
Source organism: metagenome
Primary publication:
New Thermophilic ��/�� Class Epoxide Hydrolases Found in Metagenomes From Hot Environments.
Ferrandi EE, Sayer C, De Rose SA, Guazzelli E, Marchesi C, Saneei V, Isupov MN, Littlechild JA, Monti D
Front Bioeng Biotechnol 6 144 (2018)
PMID: 30386778

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
epoxide hydrolase belonging to alpha/beta hydrolase superfamily metagenomic from Tomsk sample Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 34.02 KDa
Source organism: metagenome
Expression system: Escherichia coli

Ligands and Environments

4 bound ligands:
Ligand PEG 2 x PEG
Ligand EDO 9 x EDO
Ligand PGE 2 x PGE
Ligand IMD 1 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2221
Unit cell:
a: 41.2Å b: 84.18Å c: 157.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.198
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Biochemical and Structural Characterisation of a Novel D-Lyxose Isomerase From the Hyperthermophilic Archaeon Thermofilum sp.
De Rose et al. (2021)
6 more

4 mentions without citation

New Thermophilic α/β Class Epoxide Hydrolases Found in Metagenomes From Hot Environments.
Ferrandi et al. (2018)
3 more