X-ray diffraction
2.68Å resolution

The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with JES107

Source organism: Homo sapiens
Entry authors: Kunciw DL, Rossmann M, Stokes JE, De Fusco C, Spring DR, Hyvonen M

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Serine/threonine-protein kinase PLK1 Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 27.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P53350 (Residues: 371-603; Coverage: 39%)
Gene names: PLK, PLK1
Sequence domains: POLO box duplicated region
Structure domains: POLO box domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 33.35Å b: 91.36Å c: 35.94Å
α: 90° β: 99.71° γ: 90°
R R work R free
0.201 0.199 0.248
Expression system: Escherichia coli BL21(DE3)