› 5nec

X-ray diffraction
2.3Å resolution

Crystal structure of the siderophore receptor PiuD from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5nec/pdb
PDB 5nec coloured by chain and viewed from the front.
PDB 5nec coloured by chain and viewed from the side.
PDB 5nec coloured by chain and viewed from the top.
Source organism: Pseudomonas aeruginosa 39016
Primary publication:
TonB-Dependent Receptor Repertoire of Pseudomonas aeruginosa for Uptake of Siderophore-Drug Conjugates.
Luscher A, Moyni�� L, Auguste PS, Bumann D, Mazza L, Pletzer D, Naismith JH, K��hler T
Antimicrob. Agents Chemother. 62 (2018)
PMID: 29555629

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative outer membrane receptor for iron transport Chains: A, B
Molecule details ›
Chains: A, B
Length: 741 amino acids
Theoretical weight: 81.5 KDa
Source organism: Pseudomonas aeruginosa 39016
Expression system: Escherichia coli
UniProt:
  • Canonical: V6ALN4 (Residues: 36-766; Coverage: 100%)
Gene name: PAMH27_5021
Sequence domains:
Structure domains: TonB-dependent receptor, plug domain

Ligands and Environments

1 bound ligand:
Ligand C8E 2 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 164.522Å b: 136.452Å c: 118.955Å
α: 90° β: 132.04° γ: 90°
R-values:
R R work R free
0.224 0.223 0.249
Expression system: Escherichia coli

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Citations

3 review citations

Cefiderocol: An Overview of Its in-vitro and in-vivo Activity and Underlying Resistant Mechanisms.
Yao et al. (2021)
2 more

2 mentions without citation

Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.
Klebba et al. (2021)
1 more