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GriE in complex with manganese, succinate and (2S,4R)-5-hydroxyleucine

X-ray diffraction
1.529Å resolution
PDB entry 5ncj coloured by chain, front view.
PDB entry 5ncj coloured by chain, side view.
PDB entry 5ncj coloured by chain, top view.
Source organism: Streptomyces muensis
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Leucine hydroxylase
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Multimeric state: monomeric
Accessible surface area: 11438.61 Å2
Buried surface area: 456.54 Å2
Dissociation area: 177.89 Å2
Dissociation energy (ΔGdiss): -1.82 kcal/mol
Dissociation entropy (TΔSdiss): 1.74 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-101215
Assembly 2
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Assembly Name: Leucine hydroxylase
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Multimeric state: monomeric
Accessible surface area: 11251.41 Å2
Buried surface area: 453.85 Å2
Dissociation area: 176.36 Å2
Dissociation energy (ΔGdiss): -2.13 kcal/mol
Dissociation entropy (TΔSdiss): 1.73 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-101215

Macromolecules

Leucine hydroxylase
Chains: A, B
Length: 268 amino acids
Theoretical weight: 29.86 KDa
Source organism: Streptomyces muensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0E3URV8 (Residues: 1-265; Coverage: 98%)
Gene name: griE
Pfam: Phytanoyl-CoA dioxygenase (PhyH)
InterPro: Phytanoyl-CoA dioxygenase-like
Leucine hydroxylase in PDB entry 5ncj, assembly 1, front view.
Leucine hydroxylase in PDB entry 5ncj, assembly 1, side view.
Leucine hydroxylase in PDB entry 5ncj, assembly 1, top view.
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