› 5ncj

X-ray diffraction
1.53Å resolution

GriE in complex with manganese, succinate and (2S,4R)-5-hydroxyleucine

Released:
DOI: 10.2210/pdb5ncj/pdb
PDB 5ncj coloured by chain and viewed from the front.
PDB 5ncj coloured by chain and viewed from the side.
PDB 5ncj coloured by chain and viewed from the top.
Source organism: Streptomyces muensis
Primary publication:
Biosynthesis of methyl-proline containing griselimycins, natural products with anti-tuberculosis activity.
Lukat P, Katsuyama Y, Wenzel S, Binz T, K��nig C, Blankenfeldt W, Br��nstrup M, M��ller R
Chem Sci 8 7521-7527 (2017)
PMID: 29163906

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine hydroxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 268 amino acids
Theoretical weight: 29.86 KDa
Source organism: Streptomyces muensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0E3URV8 (Residues: 1-265; Coverage: 98%)
Gene name: griE
Sequence domains: Phytanoyl-CoA dioxygenase (PhyH)

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand SIN 2 x SIN
Ligand HL5 2 x HL5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 148.74Å b: 56.061Å c: 73.802Å
α: 90° β: 112.46° γ: 90°
R-values:
R R work R free
0.156 0.155 0.173
Expression system: Escherichia coli BL21(DE3)

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Citations

13 review citations

Metabolic engineering strategy for synthetizing trans-4-hydroxy-L-proline in microorganisms.
Zhang et al. (2021)
12 more

1 mention without citation

Biosynthesis of methyl-proline containing griselimycins, natural products with anti-tuberculosis activity.
Lukat et al. (2017)