X-ray diffraction
3.2Å resolution

Crystal structure of AcrBZ in complex with antibiotic puromycin


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Multidrug efflux pump subunit AcrB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1049 amino acids
Theoretical weight: 113.67 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:
DARPin Chains: D, E
Molecule details ›
Chains: D, E
Length: 169 amino acids
Theoretical weight: 18.32 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain
Multidrug efflux pump accessory protein AcrZ Chains: F, G, H
Molecule details ›
Chains: F, G, H
Length: 49 amino acids
Theoretical weight: 5.3 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
  • Canonical: P0AAX1 (Residues: 1-49; Coverage: 100%)
Gene names: ECs0790, Z0932, acrZ
Sequence domains: Multidrug efflux pump-associated protein AcrZ

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 147.245Å b: 167.646Å c: 249.98Å
α: 90° β: 90° γ: 90°
R R work R free
0.186 0.183 0.245
Expression system: Escherichia coli