PDBe 5nas

X-ray diffraction
2.08Å resolution

Crystal structure of human 14-3-3 zeta in complex with PI4KIIIB peptide

Released:
Source organism: Homo sapiens
Entry authors: Boura E, Eisenreichova A

Function and Biology Details

Reaction catalysed:
ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 26.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63104 (Residues: 1-230; Coverage: 94%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Phosphatidylinositol 4-kinase beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 9 amino acids
Theoretical weight: 1.1 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9UBF8 (Residues: 289-297; Coverage: 1%)
Gene names: PI4KB, PIK4CB

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 71.822Å b: 83.553Å c: 111.538Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.231
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided