› 5nak

X-ray diffraction
1.5Å resolution

Pseudomonas fluorescens kynurenine 3-monooxygenase (KMO) in complex with the enzyme substrate L-kynurenine

Released:
DOI: 10.2210/pdb5nak/pdb
PDB 5nak coloured by chain and viewed from the front.
PDB 5nak coloured by chain and viewed from the side.
PDB 5nak coloured by chain and viewed from the top.
Source organism: Pseudomonas fluorescens
Primary publication:
Structural and mechanistic basis of differentiated inhibitors of the acute pancreatitis target kynurenine-3-monooxygenase.
Hutchinson JP, Rowland P, Taylor MRD, Christodoulou EM, Haslam C, Hobbs CI, Holmes DS, Homes P, Liddle J, Mole DJ, Uings I, Walker AL, Webster SP, Mowat CG, Chung CW
Nat Commun 8 15827 (2017)
PMID: 28604669

Function and Biology Details

Reaction catalysed: 
L-kynurenine + NADPH + O(2) = 3-hydroxy-L-kynurenine + NADP(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kynurenine 3-monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 461 amino acids
Theoretical weight: 50.74 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q84HF5 (Residues: 1-461; Coverage: 100%)
Gene names: kmo, qbsG
Sequence domains: FAD binding domain
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
3 bound ligands:
Ligand CL 2 x CL
Ligand GOL 2 x GOL
Ligand KYN 1 x KYN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 69.93Å b: 52.62Å c: 137.99Å
α: 90° β: 104.07° γ: 90°
R-values:
R R work R free
0.19 0.188 0.21
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Advantages of brain penetrating inhibitors of kynurenine-3-monooxygenase for treatment of neurodegenerative diseases.
Zhang et al. (2021)
3 more

7 mentions without citation

Modulation of Enzyme Activity in the Kynurenine Pathway by Kynurenine Monooxygenase Inhibition.
Phillips et al. (2019)
6 more