› 5n9o

X-ray diffraction
1.53Å resolution

EGFP(enhanced green fluorescent protein) mutant - L232H

Released:
DOI: 10.2210/pdb5n9o/pdb
PDB 5n9o coloured by chain and viewed from the front.
PDB 5n9o coloured by chain and viewed from the side.
PDB 5n9o coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Entry authors: Kachalova GS, Popov AP, Simanovskaya AA, Krukova MV, Lipkin AV

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.98 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand AE4 2 x AE4
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 84.595Å b: 67.931Å c: 47.266Å
α: 90° β: 118.66° γ: 90°
R-values:
R R work R free
0.169 0.167 0.211
Expression system: Escherichia coli BL21(DE3)

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