› 5n8y

Electron Microscopy
4.7Å resolution

KaiCBA circadian clock backbone model based on a Cryo-EM density

Released:
DOI: 10.2210/pdb5n8y/pdb
PDB entry 5n8y coloured by chain, front view.
PDB entry 5n8y coloured by chain, side view.
PDB entry 5n8y coloured by chain, top view.
Source organism: Synechococcus elongatus
Primary publication:
Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state.
Snijder J, Schuller JM, Wiegard A, Lössl P, Schmelling N, Axmann IM, Plitzko JM, Förster F, Heck AJ
Science 355 1181-1184 (2017)
PMID: 28302852
Related structures: EMD-3602

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
PDBe Complex ID:
PDB-CPX-181279 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Circadian clock oscillator protein KaiC Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 519 amino acids
Theoretical weight: 58.07 KDa
Source organism: Synechococcus elongatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q79PF4 (Residues: 1-519; Coverage: 100%)
Gene names: Synpcc7942_1216, kaiC, see0011
Sequence domains: KaiC
Circadian clock oscillator protein KaiB Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 102 amino acids
Theoretical weight: 11.45 KDa
Source organism: Synechococcus elongatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q79PF5 (Residues: 1-102; Coverage: 100%)
Gene names: Synpcc7942_1217, kaiB, see0010
Sequence domains: KaiB domain
Circadian clock oscillator protein KaiA Chains: M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: M, N, O, P, Q, R, S, T, U, V, W, X
Length: 284 amino acids
Theoretical weight: 32.67 KDa
Source organism: Synechococcus elongatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q79PF6 (Residues: 1-284; Coverage: 100%)
Gene names: Synpcc7942_1218, kaiA, see0009
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.7Å
Relevant EMDB volumes: EMD-3602
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Orchestration of Circadian Timing by Macromolecular Protein Assemblies.
Partch CL. (2020)
10 more

4 mentions without citation

Structure, function, and mechanism of the core circadian clock in cyanobacteria.
Swan et al. (2018)
3 more