› 5n8t

X-ray diffraction
1.61Å resolution

CRYSTAL STRUCTURE OF STREPTAVIDIN D-amino acid containing peptide Gdlwqheatwkkq

Released:
DOI: 10.2210/pdb5n8t/pdb
PDB 5n8t coloured by chain and viewed from the front.
PDB 5n8t coloured by chain and viewed from the side.
PDB 5n8t coloured by chain and viewed from the top.
Source organism: Streptomyces avidinii
Primary publication:
Stepwise Evolution Improves Identification of Diverse Peptides Binding to a Protein Target.
Lyamichev VI, Goodrich LE, Sullivan EH, Bannen RM, Benz J, Albert TJ, Patel JJ
Sci Rep 7 12116 (2017)
PMID: 28935886

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 18.85 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 1-183; Coverage: 100%)
Sequence domains: Avidin family
Structure domains: Avidin-like
DLE-DTR-DGN-DHI-DGL-DAL-DTH-DTR-DLY Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.2 KDa
Source organism: Streptomyces avidinii
Expression system: Not provided

Ligands and Environments

No bound ligands
8 modified residues:
Ligand DLE 1 x DLE
Ligand DTR 2 x DTR
Ligand DGN 1 x DGN
Ligand DHI 1 x DHI
Ligand DGL 1 x DGL
Ligand DAL 1 x DAL
Ligand DTH 1 x DTH
Ligand DLY 1 x DLY

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P42212
Unit cell:
a: 57.779Å b: 57.779Å c: 84.405Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.243
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

New Immunoinformatics Tools for Swine: Designing Epitope-Driven Vaccines, Predicting Vaccine Efficacy, and Making Vaccines on Demand.
Moise et al. (2020)
1 more

1 mention without citation

Prediction of Protein Complex Structure Using Surface-Induced Dissociation and Cryo-Electron Microscopy.
Seffernick et al. (2021)