› 5n8j

X-ray diffraction
1.05Å resolution

CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE D-amino acid containing peptide GyGlanvdessG

Released:
DOI: 10.2210/pdb5n8j/pdb
PDB entry 5n8j coloured by chain, front view.
PDB entry 5n8j coloured by chain, side view.
PDB entry 5n8j coloured by chain, top view.
Source organisms:
Primary publication:
Stepwise Evolution Improves Identification of Diverse Peptides Binding to a Protein Target.
Lyamichev VI, Goodrich LE, Sullivan EH, Bannen RM, Benz J, Albert TJ, Patel JJ
Sci Rep 7 12116 (2017)
PMID: 28935886

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149732 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 183 amino acids
Theoretical weight: 18.85 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 1-183; Coverage: 100%)
Sequence domains: Avidin family
Structure domains: Avidin-like
GLY-DTY-GLY-DLE-DAL-DSG-DVA-DAS-DGL-DSN-DSN-GLY Chains: E, O, P
Molecule details ›
Chains: E, O, P
Length: 12 amino acids
Theoretical weight: 1.17 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand IPA 1 x IPA
8 modified residues:
Ligand DTY 3 x DTY
Ligand DLE 3 x DLE
Ligand DAL 3 x DAL
Ligand DSG 3 x DSG
Ligand DVA 3 x DVA
Ligand DAS 3 x DAS
Ligand DGL 3 x DGL
Ligand DSN 6 x DSN

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 46.665Å b: 84.878Å c: 57.754Å
α: 90° β: 99.1° γ: 90°
R-values:
R R work R free
0.17 0.169 0.193
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

New Immunoinformatics Tools for Swine: Designing Epitope-Driven Vaccines, Predicting Vaccine Efficacy, and Making Vaccines on Demand.
Moise et al. (2020)
1 more