› 5n8j

X-ray diffraction
1.05Å resolution

CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE D-amino acid containing peptide GyGlanvdessG

Released:
DOI: 10.2210/pdb5n8j/pdb
PDB 5n8j coloured by chain and viewed from the front.
PDB 5n8j coloured by chain and viewed from the side.
PDB 5n8j coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Stepwise Evolution Improves Identification of Diverse Peptides Binding to a Protein Target.
Lyamichev VI, Goodrich LE, Sullivan EH, Bannen RM, Benz J, Albert TJ, Patel JJ
OpenAccess logo Sci Rep 7 12116 (2017)
PMID: 28935886

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 183 amino acids
Theoretical weight: 18.85 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 1-183; Coverage: 100%)
Sequence domains: Avidin family
Structure domains: Avidin-like
GLY-DTY-GLY-DLE-DAL-DSG-DVA-DAS-DGL-DSN-DSN-GLY Chains: E, O, P
Molecule details ›
Chains: E, O, P
Length: 12 amino acids
Theoretical weight: 1.17 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand IPA 1 x IPA
8 modified residues:
Ligand DTY 3 x DTY
Ligand DLE 3 x DLE
Ligand DAL 3 x DAL
Ligand DSG 3 x DSG
Ligand DVA 3 x DVA
Ligand DAS 3 x DAS
Ligand DGL 3 x DGL
Ligand DSN 6 x DSN

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 46.665Å b: 84.878Å c: 57.754Å
α: 90° β: 99.1° γ: 90°
R-values:
R R work R free
0.17 0.169 0.193
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Peptide Folding and Binding Probed by Systematic Non-canonical Mutagenesis.
Rogers JM. (2020)
4 other articles