› 5n3r

X-ray diffraction
1.36Å resolution

cAMP-dependent Protein Kinase A from Cricetulus griseus in complex with fragment like molecule 2-(carbamoylamino)-4-methylsulfanylbutanoic acid

Released:
DOI: 10.2210/pdb5n3r/pdb
PDB 5n3r coloured by chain and viewed from the front.
PDB 5n3r coloured by chain and viewed from the side.
PDB 5n3r coloured by chain and viewed from the top.
Source organism: Cricetulus griseus
Entry authors: Siefker C, Heine A, Klebe G

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 41.19 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25321 (Residues: 1-351; Coverage: 100%)
Gene name: PRKACA
Sequence domains: Protein kinase domain

Ligands and Environments

3 bound ligands:
Ligand CDT 1 x CDT
Ligand MPD 1 x MPD
Ligand DMS 1 x DMS
2 modified residues:
Ligand SEP 3 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 51.384Å b: 71.277Å c: 97.939Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.193
Expression system: Escherichia coli BL21(DE3)

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