› 5n0r

X-ray diffraction
1.61Å resolution

Crystal structure of OphA-DeltaC6 mutant Y66F in complex with SAM

Released:
DOI: 10.2210/pdb5n0r/pdb
PDB 5n0r coloured by chain and viewed from the front.
PDB 5n0r coloured by chain and viewed from the side.
PDB 5n0r coloured by chain and viewed from the top.
Source organism: Omphalotus olearius
Primary publication:
A molecular mechanism for the enzymatic methylation of nitrogen atoms within peptide bonds.
Song H, van der Velden NS, Shiran SL, Bleiziffer P, Zach C, Sieber R, Imani AS, Krausbeck F, Aebi M, Freeman MF, Riniker S, Künzler M, Naismith JH
Sci Adv 4 eaat2720 (2018)
PMID: 30151425

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetrapyrrole methylase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 410 amino acids
Theoretical weight: 45 KDa
Source organism: Omphalotus olearius
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2R2JFH9 (Residues: 1-410; Coverage: 100%)
Sequence domains: Tetrapyrrole (Corrin/Porphyrin) Methylases

Ligands and Environments

3 bound ligands:
Ligand SAM 1 x SAM
Ligand EDO 5 x EDO
Ligand CL 2 x CL
1 modified residue:
Ligand MVA 1 x MVA

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: I222
Unit cell:
a: 86.652Å b: 93.355Å c: 165.355Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.191
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Modulating streptococcal phenotypes using signal peptide analogues.
Brennan et al. (2022)
3 more