5n0g

X-ray diffraction
1.57Å resolution

Crystal Structure of CobH T85A (precorrin-8x methyl mutase) complexed with C5 allyl-HBA

Released:
Entry authors: Nemoto-Smith EH, Lawrence AD, Brown DG, Warren MJ

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CbiC domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 21.86 KDa
Source organism: Rhodobacter capsulatus SB 1003
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: D5AV08 (Residues: 2-209; Coverage: 100%)
Gene names: RCAP_rcc02046, cobH
Sequence domains: Precorrin-8X methylmutase
Structure domains: Cobalamin biosynthesis CobH/CbiC, precorrin-8X methylmutase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 71.23Å b: 66.63Å c: 48.92Å
α: 90° β: 99.03° γ: 90°
R-values:
R R work R free
0.175 0.172 0.238
Expression system: Escherichia coli BL21