› 5mzo

X-ray diffraction
3.48Å resolution

UDP-Glucose Glycoprotein Glucosyltransferase from Chaetomium thermophilum (open conformation)

Released:
DOI: 10.2210/pdb5mzo/pdb
PDB entry 5mzo coloured by chain, front view.
PDB entry 5mzo coloured by chain, side view.
PDB entry 5mzo coloured by chain, top view.
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Primary publication:
Interdomain conformational flexibility underpins the activity of UGGT, the eukaryotic glycoprotein secretion checkpoint.
Roversi P, Marti L, Caputo AT, Alonzi DS, Hill JC, Dent KC, Kumar A, Levasseur MD, Lia A, Waksman T, Basu S, Soto Albrecht Y, Qian K, McIvor JP, Lipp CB, Siliqi D, Vasiljević S, Mohammed S, Lukacik P, Walsh MA, Santino A, Zitzmann N
Proc Natl Acad Sci U S A 114 8544-8549 (2017)
PMID: 28739903

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
UDP-glucose-glycoprotein glucosyltransferase-like protein Chain: A
Molecule details ›
Chain: A
Length: 1494 amino acids
Theoretical weight: 169.64 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Homo sapiens
UniProt:
  • Canonical: G0SB58 (Residues: 24-1505; Coverage: 100%)
Gene name: CTHT_0048990
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand NAG 1 x NAG
Ligand CA 1 x CA
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P6122
Unit cell:
a: 163.57Å b: 163.57Å c: 248.584Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.252 0.251 0.278
Expression system: Homo sapiens

Quick links

Citations

6 review citations

Protein Quality Control in the Endoplasmic Reticulum.
Adams et al. (2019)
5 more

5 mentions without citation

Calnexin cycle - structural features of the ER chaperone system.
Kozlov et al. (2020)
4 more