PDBe 5myc

X-ray diffraction
1.46Å resolution

Crystal structure of human 14-3-3 sigma in complex with LRRK2 peptide pS910

Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Leucine-rich repeat serine/threonine-protein kinase 2 Chain: P
Molecule details ›
Chain: P
Length: 38 amino acids
Theoretical weight: 4.46 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: Q5S007 (Residues: 904-941; Coverage: 2%)
Gene names: LRRK2, PARK8

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 82.517Å b: 112.411Å c: 62.689Å
α: 90° β: 90° γ: 90°
R R work R free
0.167 0.166 0.189
Expression systems:
  • Escherichia coli
  • Not provided