› 5mxc

X-ray diffraction
1.14Å resolution

Aleuria aurantia lectin (AAL) N224Q mutant in complex with alpha-methyl-L-fucoside

Released:
DOI: 10.2210/pdb5mxc/pdb
PDB entry 5mxc coloured by chain, front view.
PDB entry 5mxc coloured by chain, side view.
PDB entry 5mxc coloured by chain, top view.
Source organism: Aleuria aurantia
Primary publication:
Influence of Trp flipping on carbohydrate binding in lectins. An example on Aleuria aurantia lectin AAL.
Houser J, Kozmon S, Mishra D, Mishra SK, Romano PR, Wimmerová M, Koča J
PLoS One 12 e0189375 (2017)
PMID: 29232414

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fucose-specific lectin Chain: A
Molecule details ›
Chain: A
Length: 325 amino acids
Theoretical weight: 35.19 KDa
Source organism: Aleuria aurantia
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18891 (Residues: 1-313; Coverage: 100%)
Sequence domains: Fungal fucose-specific lectin
Structure domains: Fucose-specific lectin

Ligands and Environments

2 bound ligands:
Ligand MFU 5 x MFU
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2
Unit cell:
a: 132.25Å b: 48.63Å c: 57.58Å
α: 90° β: 103.19° γ: 90°
R-values:
R R work R free
0.138 0.138 0.146
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

N-Linked Glycan Branching and Fucosylation Are Increased Directly in Hcc Tissue As Determined through in Situ Glycan Imaging.
West et al. (2018)
3 more

2 mentions without citation

Influence of Trp flipping on carbohydrate binding in lectins. An example on Aleuria aurantia lectin AAL.
Houser et al. (2017)
1 more