› 5mwe

X-ray diffraction
2.02Å resolution

Complex between the Leucine Zipper (LZ, residues 490-567) and Centrosomin-motif 2 (CM2) domains of Drosophila melanogaster Centrosomin (Cnn)

Released:
DOI: 10.2210/pdb5mwe/pdb
PDB 5mwe coloured by chain and viewed from the front.
PDB 5mwe coloured by chain and viewed from the side.
PDB 5mwe coloured by chain and viewed from the top.
Source organism: Drosophila melanogaster
Primary publication:
Structural Basis for Mitotic Centrosome Assembly in Flies.
Feng Z, Caballe A, Wainman A, Johnson S, Haensele AFM, Cottee MA, Conduit PT, Lea SM, Raff JW
OpenAccess logo Cell 169 1078-1089.e13 (2017)
PMID: 28575671

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Centrosomin Chains: A, B
Molecule details ›
Chains: A, B
Length: 70 amino acids
Theoretical weight: 8.2 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54623 (Residues: 1254-1320; Coverage: 5%)
Gene names: Arr, CG4832, cnn
Centrosomin Chains: C, D
Molecule details ›
Chains: C, D
Length: 81 amino acids
Theoretical weight: 9.12 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54623 (Residues: 662-739; Coverage: 6%)
Gene names: Arr, CG4832, cnn

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand EDO 2 x EDO
Ligand TCE 1 x TCE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 195.99Å b: 36.5Å c: 43.78Å
α: 90° β: 102.84° γ: 90°
R-values:
R R work R free
0.23 0.228 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Centrioles and Ciliary Structures during Male Gametogenesis in Hexapoda: Discovery of New Models.
Riparbelli et al. (2020)
9 more