› 5mw8

X-ray diffraction
2.4Å resolution

INOSITOL 1,3,4,5,6-PENTAKISPHOSPHATE 2-KINASE FROM M. MUSCULUS IN COMPLEX WITH ATP and IP5

Released:
DOI: 10.2210/pdb5mw8/pdb
PDB entry 5mw8 coloured by chain, front view.
PDB entry 5mw8 coloured by chain, side view.
PDB entry 5mw8 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The crystal structure of mammalian inositol 1,3,4,5,6-pentakisphosphate 2-kinase reveals a new zinc-binding site and key features for protein function.
Franco-Echevarría E, Sanz-Aparicio J, Brearley CA, González-Rubio JM, González B
J Biol Chem 292 10534-10548 (2017)
PMID: 28450399

Function and Biology Details

Reaction catalysed: 
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol hexakisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180098 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol-pentakisphosphate 2-kinase Chain: A
Molecule details ›
Chain: A
Length: 471 amino acids
Theoretical weight: 53.85 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6P1C1 (Residues: 1-468; Coverage: 96%)
Gene name: Ippk
Sequence domains: Inositol-pentakisphosphate 2-kinase

Ligands and Environments

5 bound ligands:
Ligand ATP 1 x ATP
Ligand 5MY 1 x 5MY
Ligand ZN 1 x ZN
Ligand MG 2 x MG
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P21
Unit cell:
a: 60.162Å b: 71.495Å c: 61.197Å
α: 90° β: 111.37° γ: 90°
R-values:
R R work R free
0.243 0.241 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Inositol phosphate kinases: Expanding the biological significance of the universal core of the protein kinase fold.
Shears et al. (2019)
1 more

2 mentions without citation

The crystal structure of mammalian inositol 1,3,4,5,6-pentakisphosphate 2-kinase reveals a new zinc-binding site and key features for protein function.
Franco-Echevarría et al. (2017)
1 more