5mrw

X-ray diffraction
2.9Å resolution

Structure of the KdpFABC complex

Released:
Source organism: Escherichia coli
Primary publication:
Crystal structure of the potassium-importing KdpFABC membrane complex.
OpenAccess logo Nature 546 681-685 (2017)
PMID: 28636601

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Potassium-transporting ATPase potassium-binding subunit Chains: A, E, I
Molecule details ›
Chains: A, E, I
Length: 557 amino acids
Theoretical weight: 59.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03959 (Residues: 1-557; Coverage: 100%)
Gene names: JW0686, b0698, kdpA
Sequence domains: Potassium-transporting ATPase A subunit
Potassium-transporting ATPase ATP-binding subunit Chains: B, F, J
Molecule details ›
Chains: B, F, J
Length: 674 amino acids
Theoretical weight: 71.42 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03960 (Residues: 9-682; Coverage: 99%)
Gene names: JW0685, b0697, kdpB
Sequence domains:
Potassium-transporting ATPase KdpC subunit Chains: C, G, K
Molecule details ›
Chains: C, G, K
Length: 187 amino acids
Theoretical weight: 20.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03961 (Residues: 4-190; Coverage: 98%)
Gene names: JW0684, b0696, kdpC
Sequence domains: K+-transporting ATPase, c chain
Potassium-transporting ATPase KdpF subunit Chains: D, H, L
Molecule details ›
Chains: D, H, L
Length: 27 amino acids
Theoretical weight: 2.85 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P36937 (Residues: 1-27; Coverage: 93%)
Gene names: JW0687, b4513, kdpF

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 124.72Å b: 166.29Å c: 196.3Å
α: 90° β: 107.41° γ: 90°
R-values:
R R work R free
0.245 0.243 0.275
Expression system: Escherichia coli