5mrp

X-ray diffraction
1.9Å resolution

Arabidopsis thaliana IspD Glu258Ala mutant in complex with Azolopyrimidine (2)

Released:

Function and Biology Details

Reaction catalysed:
CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 25.37 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P69834 (Residues: 76-302; Coverage: 75%)
Gene names: At2g02500, ISPD, MCT, MECT, MEPCT, T8K22.20
Sequence domains: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R32
Unit cell:
a: 74.99Å b: 74.99Å c: 221.72Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.204 0.235
Expression system: Escherichia coli