› 5mpr

X-ray diffraction
1.6Å resolution

Single Amino Acid Variant of Human Mitochondrial Branched Chain Amino Acid Aminotransferase 2

Released:
DOI: 10.2210/pdb5mpr/pdb
PDB 5mpr coloured by chain and viewed from the front.
PDB 5mpr coloured by chain and viewed from the side.
PDB 5mpr coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Intact Protein Analysis at 21 Tesla and X-Ray Crystallography Define Structural Differences in Single Amino Acid Variants of Human Mitochondrial Branched-Chain Amino Acid Aminotransferase 2 (BCAT2).
Anderson LC, Håkansson M, Walse B, Nilsson CL
J. Am. Soc. Mass Spectrom. (2017)
PMID: 28681360

Function and Biology Details

Reaction catalysed: 
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Branched-chain-amino-acid aminotransferase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 366 amino acids
Theoretical weight: 41.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15382 (Residues: 28-392; Coverage: 93%)
Gene names: BCAT2, BCATM, BCT2, ECA40
Sequence domains: Amino-transferase class IV
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3221
Unit cell:
a: 84.032Å b: 84.032Å c: 105.446Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.131 0.128 0.179
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The mechanism of branched-chain amino acid transferases in different diseases: Research progress and future prospects.
Nong et al. (2022)
1 other article

3 mentions without citation

1H NMR-Based Metabolomics Coupled With Molecular Docking Reveal the Anti-Diabetic Effects and Potential Active Components of Berberis vernae on Type 2 Diabetic Rats.
Li et al. (2020)
2 more