› 5mp6

X-ray diffraction
1.96Å resolution

Structure of the Unliganded Fab from HIV-1 Neutralizing Antibody CAP248-2B that Binds to the gp120 C-terminus - gp41 Interface, at two Angstrom resolution.

Released:
DOI: 10.2210/pdb5mp6/pdb
PDB entry 5mp6 coloured by chain, front view.
PDB entry 5mp6 coloured by chain, side view.
PDB entry 5mp6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and Recognition of a Novel HIV-1 gp120-gp41 Interface Antibody that Caused MPER Exposure through Viral Escape.
Wibmer CK, Gorman J, Ozorowski G, Bhiman JN, Sheward DJ, Elliott DH, Rouelle J, Smira A, Joyce MG, Ndabambi N, Druz A, Asokan M, Burton DR, Connors M, Abdool Karim SS, Mascola JR, Robinson JE, Ward AB, Williamson C, Kwong PD, Morris L, Moore PL
PLoS Pathog 13 e1006074 (2017)
PMID: 28076415

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213839 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CAP248-2B Heavy Chain Chains: H, P
Molecule details ›
Chains: H, P
Length: 238 amino acids
Theoretical weight: 25.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
CAP248-2B Light Chain Chains: L, Q
Molecule details ›
Chains: L, Q
Length: 225 amino acids
Theoretical weight: 23.8 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 14 x SO4
1 modified residue:
Ligand PCA 4 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 73.003Å b: 85.903Å c: 126.277Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.24
Expression system: Homo sapiens

Quick links

Citations

6 review citations

Development of broadly neutralizing antibodies in HIV-1 infected elite neutralizers.
Landais et al. (2018)
5 more

1 mention without citation

Structure and Recognition of a Novel HIV-1 gp120-gp41 Interface Antibody that Caused MPER Exposure through Viral Escape.
Wibmer et al. (2017)