› 5mn7

X-ray diffraction
3.3Å resolution

S. aureus FtsZ 12-316 F138A GTP Closed form (3FCm)

Released:
DOI: 10.2210/pdb5mn7/pdb
PDB 5mn7 coloured by chain and viewed from the front.
PDB 5mn7 coloured by chain and viewed from the side.
PDB 5mn7 coloured by chain and viewed from the top.
Source organism: Staphylococcus aureus
Primary publication:
A Polymerization-Associated Structural Switch in FtsZ That Enables Treadmilling of Model Filaments.
Wagstaff JM, Tsim M, Oliva MA, García-Sanchez A, Kureisaite-Ciziene D, Andreu JM, Löwe J
MBio 8 (2017)
PMID: 28465423

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division protein FtsZ Chains: A, B
Molecule details ›
Chains: A, B
Length: 305 amino acids
Theoretical weight: 31.45 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A031 (Residues: 12-316; Coverage: 78%)
Gene name: ftsZ
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GTP 2 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: I222
Unit cell:
a: 116.091Å b: 130.015Å c: 134.125Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

The Search for Antibacterial Inhibitors Targeting Cell Division Protein FtsZ at Its Nucleotide and Allosteric Binding Sites.
Andreu et al. (2022)
11 more

2 mentions without citation

A Polymerization-Associated Structural Switch in FtsZ That Enables Treadmilling of Model Filaments.
Wagstaff et al. (2017)
1 more