5mm0

X-ray diffraction
2.3Å resolution

Dolichyl phosphate mannose synthase in complex with GDP-mannose and Mn2+ (donor complex)

Released:
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Structural basis for dolichylphosphate mannose biosynthesis.
OpenAccess logo Nat Commun 8 120 (2017)
PMID: 28743912

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dolichol monophosphate mannose synthase Chain: A
Molecule details ›
Chain: A
Length: 374 amino acids
Theoretical weight: 42.68 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U4M3 (Residues: 1-352; Coverage: 100%)
Gene name: PF0058
Sequence domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 90.67Å b: 146.32Å c: 95.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.258
Expression system: Escherichia coli