› 5mm0

X-ray diffraction
2.3Å resolution

Dolichyl phosphate mannose synthase in complex with GDP-mannose and Mn2+ (donor complex)

Released:
DOI: 10.2210/pdb5mm0/pdb
PDB 5mm0 coloured by chain and viewed from the front.
PDB 5mm0 coloured by chain and viewed from the side.
PDB 5mm0 coloured by chain and viewed from the top.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Structural basis for dolichylphosphate mannose biosynthesis.
Gandini R, Reichenbach T, Tan TC, Divne C
OpenAccess logo Nat Commun 8 120 (2017)
PMID: 28743912

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dolichol monophosphate mannose synthase Chain: A
Molecule details ›
Chain: A
Length: 374 amino acids
Theoretical weight: 42.68 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U4M3 (Residues: 1-352; Coverage: 100%)
Gene name: PF0058
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand GDD 1 x GDD
Ligand MN 1 x MN
Ligand CL 1 x CL
Ligand LDA 5 x LDA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 90.67Å b: 146.32Å c: 95.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.258
Expression system: Escherichia coli

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Citations

2 review citations

Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces.
Allen et al. (2019)
1 more