5mlh

X-ray diffraction
1.86Å resolution

Plantago Major multifunctional oxidoreductase in complex with 8-oxogeranial and NADP+

Released:

Function and Biology Details

Reaction catalysed:
A 3-beta-hydroxy-Delta(5)-steroid + NAD(+) = a 3-oxo-Delta(5)-steroid + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Progesterone 5-beta-reductase Chain: A
Molecule details ›
Chain: A
Length: 389 amino acids
Theoretical weight: 44.06 KDa
Source organism: Plantago major
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D6N9X1 (Residues: 1-389; Coverage: 100%)
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P43212
Unit cell:
a: 79.52Å b: 79.52Å c: 138.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.204
Expression system: Escherichia coli BL21(DE3)