X-ray diffraction
1.7Å resolution

Crystal Structure of RosB with bound intermediate AFP (8-demethyl-8-aminoriboflavin-5'-phosphate)

Source organism: Streptomyces davaonensis
Entry authors: Konjik V, Bruenle S, Demmer U, Vanselow A, Sandhoff R, Mack M, Ermler U

Function and Biology Details

Reaction catalysed:
(1a) FMN + O(2) = 8-demethyl-8-formylriboflavin 5'-phosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
8-demethyl-8-aminoriboflavin-5'-phosphate synthase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 257 amino acids
Theoretical weight: 28.9 KDa
Source organism: Streptomyces davaonensis
Expression system: Escherichia coli
  • Canonical: K4REZ6 (Residues: 1-257; Coverage: 100%)
Gene names: BN159_7989, Orf7989, rosB
Sequence domains: NADPH-dependent FMN reductase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 131.49Å b: 71.13Å c: 214.8Å
α: 90° β: 92.08° γ: 90°
R R work R free
0.176 0.174 0.213
Expression system: Escherichia coli