› 5mld

X-ray diffraction
1.7Å resolution

Crystal Structure of RosB with bound intermediate AFP (8-demethyl-8-aminoriboflavin-5'-phosphate)

Released:
DOI: 10.2210/pdb5mld/pdb
PDB 5mld coloured by chain and viewed from the front.
PDB 5mld coloured by chain and viewed from the side.
PDB 5mld coloured by chain and viewed from the top.
Source organism: Streptomyces davaonensis
Entry authors: Konjik V, Bruenle S, Demmer U, Vanselow A, Sandhoff R, Mack M, Ermler U

Function and Biology Details

Reaction catalysed: 
(1a) FMN + O(2) = 8-demethyl-8-formylriboflavin 5'-phosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
8-demethyl-8-aminoriboflavin-5'-phosphate synthase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 257 amino acids
Theoretical weight: 28.9 KDa
Source organism: Streptomyces davaonensis
Expression system: Escherichia coli
UniProt:
  • Canonical: K4REZ6 (Residues: 1-257; Coverage: 100%)
Gene names: BN159_7989, Orf7989, rosB
Sequence domains: NADPH-dependent FMN reductase

Ligands and Environments

1 bound ligand:
Ligand 8DP 8 x 8DP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 131.49Å b: 71.13Å c: 214.8Å
α: 90° β: 92.08° γ: 90°
R-values:
R R work R free
0.176 0.174 0.213
Expression system: Escherichia coli

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