5mkg

X-ray diffraction
2.44Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Diguanylate phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 256 amino acids
Theoretical weight: 28.41 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
Structure domains: EAL domain
Diguanylate phosphodiesterase Chain: B
Molecule details ›
Chain: B
Length: 254 amino acids
Theoretical weight: 28.21 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
Structure domains: EAL domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 112.23Å b: 59.4Å c: 92.78Å
α: 90° β: 115.03° γ: 90°
R-values:
R R work R free
0.24 0.238 0.285
Expression system: Escherichia coli BL21(DE3)