5mju

X-ray diffraction
3.71Å resolution

Structure of the thermostabilized EAAT1 cryst mutant in complex with the competititve inhibitor TFB-TBOA and the allosteric inhibitor UCPH101

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Excitatory amino acid transporter 1; Neutral amino acid transporter B(0) Chain: A
Molecule details ›
Chain: A
Length: 522 amino acids
Theoretical weight: 56.51 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P43003 (Residues: 1-45, 47-49, 59-61, 66-72, 76-95, 100-100, 102-104, 106-109, 114-117, 121-136, 139-140, 142-142, 144-148, 250-251, 255-255, 258-258, 260-260, 262-265, 274-277, 283-309, 311-314, 316-328, 331-335, 347-351, 353-385, 387-407, 411-421, 424-456, 459-473, 475-487, 493-508, 510-542; Coverage: 66%)
  • Canonical: Q15758 (Residues: 50-50, 54-62, 66-69, 86-88, 104-107, 109-109, 113-113, 118-121, 126-128, 145-146, 149-149, 151-151, 157-237, 240-242, 244-245, 247-247, 249-249, 254-261, 266-270, 298-298, 303-303, 317-318, 324-334, 340-340, 374-374, 396-398, 410-411, 445-446, 462-462, 476-480, 498-498; Coverage: 31%)
Gene names: ASCT2, EAAT1, GLAST, GLAST1, M7V1, RDR, RDRC, SLC1A3, SLC1A5

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P63
Unit cell:
a: 124.33Å b: 124.33Å c: 90.81Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.227 0.254
Expression system: Homo sapiens