X-ray diffraction
2Å resolution

Crystal Structure of Lactococcus lactis Thioredoxin Reductase (FO conformation)


Function and Biology Details

Reaction catalysed:
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Thioredoxin reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 327 amino acids
Theoretical weight: 36.03 KDa
Source organism: Lactococcus lactis subsp. cremoris
Expression system: Escherichia coli BL21(DE3)
  • Canonical: A2RLJ5 (Residues: 1-308; Coverage: 100%)
Gene names: llmg_1588, trxB1
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase

Ligands and Environments

Cofactor: Ligand FAD 4 x FAD
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P21
Unit cell:
a: 73.61Å b: 132.26Å c: 73.5Å
α: 90° β: 112.62° γ: 90°
R R work R free
0.232 0.23 0.276
Expression system: Escherichia coli BL21(DE3)