› 5miy

X-ray diffraction
1.72Å resolution

Crystal structure of the E3 ubiquitin ligase RavN from Legionella pneumophila

Released:
DOI: 10.2210/pdb5miy/pdb
PDB entry 5miy coloured by chain, front view.
PDB entry 5miy coloured by chain, side view.
PDB entry 5miy coloured by chain, top view.
Source organism: Legionella pneumophila
Primary publication:
RavN is a member of a previously unrecognized group of Legionella pneumophila E3 ubiquitin ligases.
Lin YH, Lucas M, Evans TR, Abascal-Palacios G, Doms AG, Beauchene NA, Rojas AL, Hierro A, Machner MP
PLoS Pathog 14 e1006897 (2018)
PMID: 29415051

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166000 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin ligase RavN Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 128 amino acids
Theoretical weight: 14.55 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

3 bound ligands:
Ligand SO4 13 x SO4
Ligand EDO 5 x EDO
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: C222
Unit cell:
a: 114.16Å b: 177.01Å c: 79.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.161 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Pathogenicity and Virulence of Legionella: Intracellular replication and host response.
Chauhan et al. (2021)
9 more

1 mention without citation

RavN is a member of a previously unrecognized group of Legionella pneumophila E3 ubiquitin ligases.
Lin et al. (2018)