› 5miv

X-ray diffraction
3.1Å resolution

G307E variant of murine Apoptosis Inducing Factor in complex with NAD+

Released:
DOI: 10.2210/pdb5miv/pdb
PDB entry 5miv coloured by chain, front view.
PDB entry 5miv coloured by chain, side view.
PDB entry 5miv coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural bases of the altered catalytic properties of a pathogenic variant of apoptosis inducing factor.
Sorrentino L, Cossu F, Milani M, Aliverti A, Mastrangelo E
Biochem Biophys Res Commun 490 1011-1017 (2017)
PMID: 28666871

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apoptosis-inducing factor 1, mitochondrial Chains: A, C
Molecule details ›
Chains: A, C
Length: 535 amino acids
Theoretical weight: 58.4 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Z0X1 (Residues: 78-612; Coverage: 87%)
Gene names: Aif, Aifm1, Pdcd8
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NAD 2 x NAD
Ligand FAD 2 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 73.43Å b: 116.78Å c: 166.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.263
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Alpha synuclein post translational modifications: potential targets for Parkinson's disease therapy?
Brembati et al. (2023)
2 more