› 5mis

X-ray diffraction
1.81Å resolution

Crystal Structure of Lactococcus lactis Thioredoxin Reductase Exposed to Visible Light (180 min)

Released:
DOI: 10.2210/pdb5mis/pdb
PDB entry 5mis coloured by chain, front view.
PDB entry 5mis coloured by chain, side view.
PDB entry 5mis coloured by chain, top view.
Source organism: Lactococcus cremoris
Primary publication:
The structure of Lactococcus lactis thioredoxin reductase reveals molecular features of photo-oxidative damage.
Skjoldager N, Blanner Bang M, Rykær M, Björnberg O, Davies MJ, Svensson B, Harris P, Hägglund P
Sci Rep 7 46282 (2017)
PMID: 28397795

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106949 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 36.03 KDa
Source organism: Lactococcus cremoris
Expression system: Escherichia coli
UniProt:
  • Canonical: A2RLJ5 (Residues: 1-308; Coverage: 100%)
Gene names: llmg_1588, trxB1
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand NAP 1 x NAP
2 bound ligands:
Ligand PEG 2 x PEG
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P41212
Unit cell:
a: 120.92Å b: 120.92Å c: 60.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.232
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Photo-Oxidation of Therapeutic Protein Formulations: From Radical Formation to Analytical Techniques.
Hipper et al. (2021)
2 other articles

1 mention without citation

The structure of Lactococcus lactis thioredoxin reductase reveals molecular features of photo-oxidative damage.
Skjoldager et al. (2017)