› 5mil

X-ray diffraction
2.1Å resolution

Pirating conserved phage mechanisms promotes promiscuous staphylococcal pathogenicity islands transfer.

Released:
DOI: 10.2210/pdb5mil/pdb
PDB 5mil coloured by chain and viewed from the front.
PDB 5mil coloured by chain and viewed from the side.
PDB 5mil coloured by chain and viewed from the top.
Source organism: Staphylococcus aureus
Primary publication:
Pirating conserved phage mechanisms promotes promiscuous staphylococcal pathogenicity island transfer.
Bowring J, Neamah MM, Donderis J, Mir-Sanchis I, Alite C, Ciges-Tomas JR, Maiques E, Medmedov I, Marina A, Penadés JR
OpenAccess logo Elife 6 (2017)
PMID: 28826473

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ORF026 Chains: A, B
Molecule details ›
Chains: A, B
Length: 202 amino acids
Theoretical weight: 22.9 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q4ZDP4 (Residues: 1-168; Coverage: 100%)
Sequence domains: dUTPase

Ligands and Environments

2 bound ligands:
Ligand DUP 2 x DUP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P21
Unit cell:
a: 33.47Å b: 88.84Å c: 54.15Å
α: 90° β: 91.68° γ: 90°
R-values:
R R work R free
0.189 0.186 0.244
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Phage satellites and their emerging applications in biotechnology.
Ibarra-Chávez et al. (2021)
7 other articles

2 mentions without citation

HDX and Native Mass Spectrometry Reveals the Different Structural Basis for Interaction of the Staphylococcal Pathogenicity Island Repressor Stl with Dimeric and Trimeric Phage dUTPases.
Nyíri et al. (2019)
1 more