5mhg

X-ray diffraction
2.8Å resolution

Crystal structure of anti-T4 Fab fragment with tetraiodoBPA

Released:
Source organism: Mus musculus
Entry authors: Parkkinen T, Rouvinen J

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fab-Fragment Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.32 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab-Fragment Chain: H
Molecule details ›
Chain: H
Length: 235 amino acids
Theoretical weight: 25.37 KDa
Source organism: Mus musculus
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 131.767Å b: 56.992Å c: 61.993Å
α: 90° β: 98.39° γ: 90°
R-values:
R R work R free
0.224 0.221 0.291
Expression system: Escherichia coli