› 5mhg

X-ray diffraction
2.8Å resolution

Crystal structure of anti-T4 Fab fragment with tetraiodoBPA

Released:
DOI: 10.2210/pdb5mhg/pdb
PDB 5mhg coloured by chain and viewed from the front.
PDB 5mhg coloured by chain and viewed from the side.
PDB 5mhg coloured by chain and viewed from the top.
Source organism: Mus musculus
Entry authors: Parkkinen T, Rouvinen J

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fab-Fragment Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.32 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab-Fragment Chain: H
Molecule details ›
Chain: H
Length: 235 amino acids
Theoretical weight: 25.37 KDa
Source organism: Mus musculus
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand 4IB 1 x 4IB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 131.767Å b: 56.992Å c: 61.993Å
α: 90° β: 98.39° γ: 90°
R-values:
R R work R free
0.224 0.221 0.291
Expression system: Escherichia coli

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