› 5mha

X-ray diffraction
1.57Å resolution

D-2-hydroxyacid dehydrogenases (D2-HDH) from Haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 A resolution)

Released:
DOI: 10.2210/pdb5mha/pdb
PDB entry 5mha coloured by chain, front view.
PDB entry 5mha coloured by chain, side view.
PDB entry 5mha coloured by chain, top view.
Source organism: Haloferax mediterranei ATCC 33500
Entry authors: Bisson C, Baker PJ, Domenech Perez J, Pramanpol N, Harding SE, Rice DW, Ferrer J

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174099 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-2-hydroxyacid dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 308 amino acids
Theoretical weight: 33.37 KDa
Source organism: Haloferax mediterranei ATCC 33500
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2VEQ7 (Residues: 1-308; Coverage: 100%)
Gene names: HFX_2024, ddh, serA5
Sequence domains: D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

6 bound ligands:
Ligand MG 7 x MG
Ligand EDO 8 x EDO
Ligand 7N5 2 x 7N5
Ligand NDP 2 x NDP
Ligand SO4 1 x SO4
Ligand 7N6 1 x 7N6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 62.54Å b: 76.3Å c: 66.99Å
α: 90° β: 97.04° γ: 90°
R-values:
R R work R free
0.176 0.174 0.216
Expression system: Escherichia coli BL21(DE3)

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