5mgh

X-ray diffraction
1.87Å resolution

Crystal structure of pathogenic mutants of human mitochodnrial PheRS

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine--tRNA ligase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 405 amino acids
Theoretical weight: 47.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95363 (Residues: 47-451; Coverage: 90%)
Gene names: FARS1, FARS2, HSPC320
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 55.045Å b: 89.355Å c: 97.226Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.23
Expression system: Escherichia coli