› 5mfd

X-ray diffraction
2.3Å resolution

Designed armadillo repeat protein YIIIM''6AII in complex with pD_(KR)5

Released:
DOI: 10.2210/pdb5mfd/pdb
PDB 5mfd coloured by chain and viewed from the front.
PDB 5mfd coloured by chain and viewed from the side.
PDB 5mfd coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Structures of designed armadillo repeat proteins binding to peptides fused to globular domains.
Hansen S, Kiefer JD, Madhurantakam C, Mittl PRE, Pl��ckthun A
Protein Sci. (2017)
PMID: 28691351

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
YIIIM''6AII Chains: A, C, E, G, I, J, K, L
Molecule details ›
Chains: A, C, E, G, I, J, K, L
Length: 328 amino acids
Theoretical weight: 34.35 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12
Capsid decoration protein Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 109 amino acids
Theoretical weight: 11.48 KDa
Source organism: Lambdavirus lambda
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P03712 (Residues: 21-110; Coverage: 82%)
Gene names: D, lambdap07
Sequence domains: Bacteriophage lambda head decoration protein D
Structure domains: Head decoration protein D

Ligands and Environments

1 bound ligand:
Ligand CA 49 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P63
Unit cell:
a: 194.66Å b: 194.66Å c: 241.74Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.19 0.214
Expression system: Escherichia coli K-12

Quick links

Citations

2 citation in other articles

An automated iterative approach for protein structure refinement using pseudocontact shifts.
Cucuzza et al. (2021)
1 more

1 mention without citation

Structures of designed armadillo repeat proteins binding to peptides fused to globular domains.
Hansen et al. (2017)