› 5mf1

X-ray diffraction
3.3Å resolution

Crystal structure of a C-terminally truncated trimeric ectodomain of the Chlamydomonas reinhardtii gamete fusion protein HAP2

Released:
DOI: 10.2210/pdb5mf1/pdb
PDB 5mf1 coloured by chain and viewed from the front.
PDB 5mf1 coloured by chain and viewed from the side.
PDB 5mf1 coloured by chain and viewed from the top.
Source organism: Chlamydomonas reinhardtii
Primary publication:
The Ancient Gamete Fusogen HAP2 Is a Eukaryotic Class II Fusion Protein.
Fédry J, Liu Y, Péhau-Arnaudet G, Pei J, Li W, Tortorici MA, Traincard F, Meola A, Bricogne G, Grishin NV, Snell WJ, Rey FA, Krey T
OpenAccess logo Cell 168 904-915.e10 (2017)
PMID: 28235200

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hapless 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 611 amino acids
Theoretical weight: 64.42 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: A4GRC6 (Residues: 23-592; Coverage: 51%)
Gene names: GCS1, HAP2

Ligands and Environments

2 bound ligands:
Ligand NAG 3 x NAG
Ligand NGA 3 x NGA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P212121
Unit cell:
a: 103.97Å b: 114.06Å c: 137.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.266
Expression system: Drosophila melanogaster

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Citations

14 review citations

Structural Insights into Membrane Fusion Mediated by Convergent Small Fusogens.
Yang et al. (2021)
13 more