› 5me6

X-ray diffraction
2.9Å resolution

Crystal Structure of eiF4E from C. melo bound to a CAP analog

Released:
DOI: 10.2210/pdb5me6/pdb
PDB 5me6 coloured by chain and viewed from the front.
PDB 5me6 coloured by chain and viewed from the side.
PDB 5me6 coloured by chain and viewed from the top.
Source organism: Cucumis melo
Primary publication:
Structure of eIF4E in Complex with an eIF4G Peptide Supports a Universal Bipartite Binding Mode for Protein Translation.
Miras M, Truniger V, Silva C, Verdaguer N, Aranda MA, Querol-Audí J
Plant Physiol. 174 1476-1491 (2017)
PMID: 28522457

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 4E-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 186 amino acids
Theoretical weight: 21.35 KDa
Source organism: Cucumis melo
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00LS8 (Residues: 53-235; Coverage: 78%)
Gene names: E6C27_scaffold96G002720, eIF4E
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e

Ligands and Environments

1 bound ligand:
Ligand M7G 2 x M7G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 57.699Å b: 108.868Å c: 122.839Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.263
Expression system: Escherichia coli

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Citations

5 review citations

Control of the eIF4E activity: structural insights and pharmacological implications.
Romagnoli et al. (2021)
4 more