› 5mdo

X-ray diffraction
1.95Å resolution

Crystal structure of in vitro folded Chitoporin VhChip from Vibrio harveyi (crystal form I)

Released:
DOI: 10.2210/pdb5mdo/pdb
PDB 5mdo coloured by chain and viewed from the front.
PDB 5mdo coloured by chain and viewed from the side.
PDB 5mdo coloured by chain and viewed from the top.
Source organism: Vibrio harveyi
Primary publication:
Structural basis for chitin acquisition by marine Vibrio species.
Aunkham A, Zahn M, Kesireddy A, Pothula KR, Schulte A, Basl�� A, Kleinekath��fer U, Suginta W, van den Berg B
Nat Commun 9 220 (2018)
PMID: 29335469

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Porin domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 352 amino acids
Theoretical weight: 39.19 KDa
Source organism: Vibrio harveyi
Expression system: Escherichia coli
UniProt:
  • Canonical: L0RVU0 (Residues: 26-375; Coverage: 100%)
Gene name: chiP
Sequence domains: Gram-negative porin

Ligands and Environments

2 bound ligands:
Ligand NA 18 x NA
Ligand C8E 17 x C8E
1 modified residue:
Ligand MSE 60 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 146.884Å b: 123.379Å c: 147.026Å
α: 90° β: 117.89° γ: 90°
R-values:
R R work R free
0.167 0.167 0.187
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Role of the lipid bilayer in outer membrane protein folding in Gram-negative bacteria.
Horne et al. (2020)
1 more

5 mentions without citation

Structural basis for chitin acquisition by marine Vibrio species.
Aunkham et al. (2018)
4 more